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Evaluating the effect of aquatic extraction of Cannabis sativa seed on spatial memory consolidation in rats


The existence of an endocannabinoid system in the central nervous system that consists of G protein-coupled CB1 cannabinoid receptors and endocannabinoids, including arachidonylethanolamide and 2-arachidonoylglycerol, has gained general acceptance. Recent reports suggest that this system may serve several physiological functions including learing and memory functions

Materials and methods

40 male wistar rats (3-4 month, 320-260 g) were completely divided into 4 experimental groups and control group. Cannabis sativa seed was extracted with Soxhlet apparatus. To test spatial memory, Morris water mazemaze (7 days,4 trails) was used. Experimental groups with 50, 100, 150 -1,210 were injected in the peritoneal (IP) orderly and after one hour of injection spatial memory was done.


The result show that experimental groups (50,100,150 -1 doses), for learning time have significant level deduction in the comparison of controlgroup (p < 0.05), but experimental group with 210 dose has not significant level in the comparison of control group (p < 0.05).


We demonstrate tetrahydrocannabinol can change brain function as learning and memory processes and probably was done with Depolarization-Induced Suppression of excitatory (DSE) mechanism in the CA1 area of Hippocamp that with neurotransmitter regulation cause to neuroplasticity.


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We thank Dr Heravi and Mr Kazemi for money support and Azad University of Mashhad for prepare Lab for Test.

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Correspondence to Saeedeh Ebrahimpour.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ebrahimpour, S., Tehranipour, M., Behnam, M. et al. Evaluating the effect of aquatic extraction of Cannabis sativa seed on spatial memory consolidation in rats. Ann Gen Psychiatry 9, S139 (2010).

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  • Aquatic Extraction
  • Brain Function
  • Spatial Memory
  • Memory Process
  • Memory Function