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Evaluating the effect of Tetrahydrocannabinol (∆9-THC) extracted from Cannabis sativa plant on spatial memory consolidation in rats


As the point of physiology, memories form changes in the conducting message in the neural webs. These changes cause the formation of long-term potentiation. Δ9-THC is a psychotropic component of Cannabis sativa plant. Studies show this matter can bind Cannabinoid receptor in CA1 area of Hippocamp. Thus the aim of this study is to evaluate the effect of aqua extraction Cannabis sativa seed on spatial memory consolidation in rats.

Materials and methods

A number of 40 male wistar rats (3-4 month, 320-260 g) were completely divided into 4 experimental groups and control group. Cannabis sativa seed was extracted with Soxhlet apparatus. To test spatial memory, Morris water mazemaze (7 days, 4 trails) was used.

Experimental groups with 50, 100, 150 -1,210 were injected in the peritoneal (IP) and after one hour of injection spatial memory was scaled.


The result show that experimental groups (50,100,150 doses), for learning time have significant level eduction in the comparison of control group (p < 0.05), but experimental group with 210 dose has not significant level in the comparison of control group (p < 0.05).


We demonstrate tetrahydrocannabinol can change brain function as learning and memory processes and probably was done with Depolarization-Induced Suppression of excitatory (DSE) mechanism in the CA1 area of Hippocamp that with neurotransmitter regulation cause europlasticity.


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The authors are grateful to Islamic Azad University of Mashhad, Iran for support.

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Correspondence to Saeedeh Ebrhimpour.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ebrhimpour, S., Kafaee, M., Tehraniour, M. et al. Evaluating the effect of Tetrahydrocannabinol (∆9-THC) extracted from Cannabis sativa plant on spatial memory consolidation in rats. Ann Gen Psychiatry 9, S128 (2010).

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  • Public Health
  • Level Eduction
  • Aqua Extraction
  • Brain Function
  • Spatial Memory